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Mistake 1: Shooting water directly onto the powder This is the most common error

The pKas of the compound are 10.3,7.8, and 6.2.[1] Synthesis of Ipamorelin In particular, ipamorelin was prepared from a total of 4.53 g of the peptide resin Boc-Aib-His (Trt)-D-2Nal-DPhe-Lys (Boc)-NH-resin according to the Fmoc strategy on an Applied Biosystems 431A peptide synthesizer in two identical runs (to obtain sufficient material) in 1 mmol scale using the FastMoc UV protocols supplied by the manufacturer, that employ HBTU mediated couplings in NMP and UV monitoring of the deprotection of the Fmoc protection group

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Trent J, Krisner R